Olga Zolotareva and Konstantinos Tzanakis
Seminar: Monday 10:00-12:00
Classroom: U10-146
In this seminar, students will choose, study and present one of the recent bioinformatic software tools published in peer-reviewed journals. The students are encouraged to suggest topics and tools of their interest or choose one of the following topics (shortlists of tools and a review article for topics will be provided):
Topic | Review | Recommended tools |
- Noise filtering and Baseline Correction - Peak detection - Metabolite identification - Metabolomics data pre-treatment (normalization, scaling, transformation) | Piasecka et al., 2019 Spicer et al., 2017 Lazar et al., 2015 | OpenMs, XCMS, Workflow4Metabolomics, MeltDB, MetAlign |
Peak alignment | Lange et al., 2008 | XCMS, OpenMs, MZmine |
De novo transcriptome assembly | Hölzer & Marz, 2019 | Trinity, SPAdes |
Read Mapping | Canzar & Salzberg, 2015 Reinert et al., 2015 | bwa, |
| Xu 2018 | |
Expression Quantification and differential expression analysis | Lowe et al., 2017 Finotello & Di Camillo, 2016 | kallisto, limma-voom, |
Biclustering | Pontes et al., 2015 | |
Gene prioritization | Zolotareva & Kleine 2019 | Endeavour, |
The participants will give oral presentations (20-45 min’s, optimally about 25 min's) and prepare short summaries (5 to 10 pages) based on original research papers. In their reports, students must (i) briefly explain the problem solved by a selected tool (ii) describe the algorithm(s) implemented in this tool (iii) explain the choice of the tool among its analogs. At the end of the course, the students will peer-review their essays and revise them based on the received feedback.
In this seminar students will develop skills necessary for:
Title | Date |
Preliminaries, topic selection (Kostas) | 07.10.2019 |
Example presentation (Olga) | 14.10.2019 |
Best Practices for Scientific Writing (Kostas) | 21.10.2019 |
(preparing presentations) | 28.10.2019 |
Jonas Waas - De novo transcriptome assembly, SPAdes | 04.11.2019 |
Aya Bezine - Single Nucleotide Variant Calling, VarScan2 | 11.11.2019 |
Fabian Kolesch - Biclustering, QUBIC | 18.11.2019 |
(preparing reports) | 25.11.2019 |
(preparing reports) | 02.12.2019 |
Lukas Schulte - Expression quantification and DE analysis,DeSeq2 | 16.12.2019 |
Submitting reports (1st version) | 20.12.2019 |
Submitting reviews of essays | 13.01.2020 |
Submitting revised essays | 20.01.2020 |